The thesis discusses the topic of volatile organic compounds in Norway spruce (Picea abies, L.) and its ecotypes. It comprises the characteristic of Norway spruce, its importance in the industry and it gives a brief overview of the current position with spruce management during ongoing climate change. The thesis offers the characteristic of organic volatile compounds, the question of stress of conifers and it defines different ways of defence mechanisms against stress and pathogens. The experiment is dealing with volatile compounds profiles within 3 different ecotypes of Norway spruce – acuminata (low), europaea (medium) and obovata (high).
Experiment was held in the study site near Cukrak (West of Prague) where the clone archive could be found. It contains all 3 ecotypes cited above in age 51 years (2020). The ability of producing volatile compounds in trees was induced by application of methyl jasmonate between the layer of phloem and wood to the studied trees. The samples of bark and phloem were collected for further laboratory processing after 3 weeks from the MeJa application. More than 400 samples were taken during spring and summer sampling. Extractable substances were identified with GS-MS.
The profiles of extracted volatile compounds were processed chemometrically in order to identify them and moreover determine, which ecotype they belong to. In addition to non-target analysis, some compounds were quantified and results from the quantity were used in order to find out differences within ecotypes. Compounds responsible for separation between control and treated samples were identified. In case of ecotypes, differences were found in quantity of estragole and 3-caren.
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